pH Electrodes Designed and Manufactured by Hanna Calibration pH Electrode Preparation Procedure A clean, conditioned Hanna pH electrode will provide the best measurements possible. When using a new electrode, remove the protective bulb cap and inspect the electrode. As water may have evaporated during shipping or storage, salt crystals may be found in and around the protective cap or on the pH bulb, this is normal . Rinse off with water. During transport, air bubbles may have formed inside the glass bulb. Shake down the electrode as you would with a spirit filled thermometer. Condition the sensing tip; soak the pH bulb and junction in HI70300 storage solution for at least one hour or longer. If possible, an overnight soak is best. This will hydrate a dehydrated glass sensor and thoroughly wet a dried reference junction. Junction pH Bulb Rinse Electrode with Purified Water Prior to placing the electrode in calibration solution, it should be thoroughly rinsed with clean, purified water to prevent any contamination to the pH buffer. The electrode should always be rinsed with purified water after placing it in any solution. Use Fresh pH Buffer for Calibration The calibration of the pH electrode is only as good as the buffer used. Once a bottle of buffer is open, it should be discarded after six months of use. To prevent cross-contamination, never pour buffer back into the bottle. If the same buffer is to be used for multiple calibrations, it is better to pour a small amount of buffer in a separate container that can be sealed. If using a separate container, the buffer should be changed frequently (i.e. daily, weekly). It is important to note that pH buffers at higher values (i.e. pH 10.01) are less stable than lower values, this is due to atmospheric CO2 diffusing into the buffer, forming carbonic acid. If the buffer is old, the actual value might be less than stated on the bottle, resulting in a low slope. Open Reference Fill Cap on Refillable Electrodes If using a refillable pH electrode, the fill cap should be removed prior to calibration and measurement. Removing the cap creates positive head pressure in the reference cell allowing for higher flow rate of electrolyte through the outer junction. A higher flow rate will result in a faster and more stable reading. Submerse Electrode Past Junction It is critical that the junction of the electrode be completely submersed in the pH buffer or sample. Failure to do so will result in erratic readings. Use a Magnetic Stirrer For benchtop meters, it is beneficial to use a magnetic stirrer. A magnetic stirrer will ensure that the pH buffer or sample is homogenous. The movement of the solution will also increase the response time of the electrode in the solution. One-point Calibration For one-point calibration it is important to calibrate the pH electrode in pH 7.0. This calibration determines the offset value. The mV value at pH 7.00 ideally should be 0.0. Multiple-point Calibration For improved accuracy it is recommend to calibrate a minimum of two points. The second point determines the slope of the line. It is important to use buffers that bracket the expected value of the sample to be tested. For example, if the expected value is pH 8, the electrode should be calibrated using pH 7.01 and pH 10.01 buffer. Electrode Fill Solutions The electrolyte level in refillable electrodes should be checked before performing any calibration. If the level is low (1 cm or ½” below fill hole), refill with the proper electrolyte solution to ensure the optimum electrode performance. This simple maintenance step helps guarantee adequate head pressure to promote efficient and precise reading. Always use the appropriate fill solution for your pH electrode. Typically single junction pH electrodes use the HI7071 electrolyte solution (3.5M KCl + AgCl) while double junction pH electrodes use HI7082 electrolyte solution (3.5M KCl). pH mV 4 10 7 +177.48 - 177.48 pH mV 4 10 7 Refill with Proper Electrolyte Below this Level Good Electrolyte Level 1/2” Reference Fill Cap Offset Slope 2 pH 2.129 www.hannainst.com | electrodes
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